Current Issue

Volume 13, Number 2, Jul-Sep 2019 Pages: 154-160

The Effect of Diazinon on Cell Proliferation and Apoptosis in Testicular Tissue of Rats and The Protective Effect of Vitamin E


Fatemeh Rahimi Anbarkeh, M.Sc, 1, Mohammad Reza Nikravesh, Ph.D, 1, *, Mehdi Jalali, Ph.D, 1, Hamid Reza Sadeghnia, Ph.D, 2, Zinat Sargazi, M.Sc, 3,
Department of Anatomy and Cell Biology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Department of Pharmacology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Research Center for Neuroscience, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran
*Corresponding Address: P.O.Box: 9177948564 Department of Anatomy and Cell Biology Faculty of Medicine Mashhad University of Medical Sciences Mashhad Iran Email:nikraveshmr@mums.ac.ir

Abstract

Background

Diazinon (DZN) is an organophosphate pesticide, and nowadays this pesticide is mostly used in agri- culture. In this study, we analyzed the effects of DZN and vitamin E (Vit E) on apoptosis and the proliferation of germ cells in rat testis.

Materials and Methods

In this experimental study, 30 male Wistar rats were divided into five groups (n=6 per group) consisting of control, sham (received olive oil), experimental group i (60 mg/kg DZN), experimental group ii (60 mg/kg DZN and 200 mg/kg Vit E), and experimental group iii (200 mg/kg Vit E). After six weeks, left testis of rats was removed for the detection of proliferative cell nuclear antigen (PCNA) and terminal deoxynucleotidyl transferase end-labeling (TUNEL).

Results

Compared with the control group, DZN in the experimental group i decreased the number of PCNA-positive cells and increased the number of TUNEL-positive cells (P<0.001). Vit E improved detrimental changes by the de- crease in the rate of apoptosis and the increase in the proliferation of testicular germ cells (P<0.001).

Conclusion

Vit E can decrease the number of TUNEL-positive cells and increase the number of PCNA-positive cells by the neutralization of the toxicity caused by DZN in the testicular tissue.