Current Issue

Volume 13, Number 1, Apr-Jun 2019 Pages: 45-50

Comparing The Effects of Glycyrrhiza glabra Root Extract, A Cyclooxygenase-2 Inhibitor (Celecoxib) and A Gonadotropin-Releasing Hormone Analog (Diphereline) In A Rat Model of Endometriosis


Bahia Namavar Jahromi, M.D, 1, 2, *, Farnaz Farrokhnia, M.D, 2, 3, Nader Tanideh, Ph.D, 4, 5, Perikala Vijayananda Kumar, M.D, 6, Mohammad Ebrahim Parsanezhad, M.D, 1, 2, Sanaz Alaee, Ph.D, 7, *,
Infertility Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Department of Obstetrics and Gynecology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
Student Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Stem Cell Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Department of Pharmacology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
Department of Pathology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
Department of Reproductive Biology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran
*Corresponding Addresses: P.O.Box: 7134844119 Infertility Research Center Shiraz University of Medical Sciences Shiraz Iran P.O.Box: 7133654361 Department of Reproductive Biology School of Advanced Medical Sciences and Technologies Shiraz University of Medical Sciences Shiraz Iran Emails:namavarb@sums.ac.ir,alaee@sums.ac.ir

Abstract

Background

The purpose of this study was to compare the effects of Glycyrrhiza glabra (Licorice), a cyclooxyge- nase-2 inhibitor (Celecoxib) and a gonadotropin-releasing hormone analog (Diphereline®), with a control group on endometrial implants in rats.

Materials and Methods

In this experimental study, endometriosis was induced in rats by auto transplantation and after confirmation, the rats were divided into 4 groups that were treated for 6 weeks with normal saline (0.5 ml/day, orally), licorice extract (3000 mg/kg/day, orally), celecoxib (50 mg/kg, twice a day, orally) or diphereline (3 mg/kg, intramuscularly). At the end of treatments, the mean area, volume, histopathology and hemosiderin-laden macrophage (HLM) counts of the endometrial implants were evaluated and compared among the four groups.

Results

The mean area, volume and HLM counts of the implants in the licorice group were significantly lower than those of the control group (P<0.001). The histopathologic grades of endometrial implants were significantly decreased by licorice compared to the control group (P<0.001). There was no significant change in the mentioned parameters in rats treated with celecoxib compared to the control group. Diphereline was the most potent agent for suppressing the growth of endometrial implants in terms of all of the above-mentioned parameters.

Conclusion

Licorice decreased the growth and histopathologic grades of auto-transplanted endometrial implants. However, while celcoxib had no significant effect, diphereline showed the highest potency for decreasing the endome- trial growth. Licorice may have the potential to be used as an alternative medication for the treatment of endometriosis.