Current Issue

Volume 12, Number 4, Jan-Mar 2019 Pages: 335-338

Expression of Ghrelin and Its Receptor mRNA in Bovine Oocyte and Cumulus Cells


Matías Angel Sirini, V.M.S, 1, Juan Patricio Anchordoquy, Ph.D, 1, Silvina Quintana, Ph.D, 2, Cecilia Furnus, Ph.D, 1, Alejandro Enrique Relling, Ph.D, 1, Juan Mateo Anchordoquy, Ph.D, 1, *,
IGEVET-Institute of Veterinary Genetic “Prof. Fernando N. Dulout” (UNLP-CONICET LA PLATA), Faculty of Veterinary Sciences, National University of La Plata, La Plata, Buenos Aires, Argentina
Bee Reasearch Center, Department of Biology, FCEy N, National University of Mar del Plata - CONICET, Mar del Plata, Buenos Aires, Argentina
*Corresponding Address: IGEVET-Institute of Veterinary Genetic “Prof. Fernando N. Dulout” (UNLP-CONICET LA PLATA) Faculty of Veterinary Sciences National University of La Plata La Plata Buenos Aires Argentina Email:mateoanchordoquy@fcv.unlp.edu.ar

Abstract

Energy balance is regulated by ghrelin which is a neuroendocrine modulator. Ghrelin is expressed in repro- ductive organs. However, the role of ghrelin during in vitro maturation (IVM) and bovine preimplantational development is limited. The purpose of this study was to measure the expression of ghrelin (GHRL) and its receptor growth hormone secretagogue receptor 1A (GHS-R1A) mRNA, and determine cumulus oocyte complex (COC) viability after IVM with 0, 20, 40 and 60 pM of ghrelin. Also, pronuclear formation was recorded after in vitro fertilization (IVF). GHRL and GHS-R1A mRNA expression in oocyte and cumu- lus cells (CCs) was assessed using reverse transcription-polymerase chain reaction (PCR). Oocyte and CC viability were analyzed with the fluorescein diacetate fluorochrome-trypan blue technique. Pronuclear formation was determined 18 hours after IVF with Hoechst 33342. The results demonstrated that ghrelin mRNA is present in oocyte and CCs before and after 24 hours IVM with all treatments. Ghrelin receptor, GHS-R1A, was only detected in oocytes and CCs after 24 hours IVM with 20, 40 and 60 pM of ghrelin. Oocyte viability was not significantly different (P=0.77) among treatments. However, CC viability was significantly lower (P=0.04) when COCs were matured with ghrelin (77.65, 72.10, 66.32 and 46.86% for 0, 20, 40, and 60 pM of ghrelin, respectively). The chance of two pronuclei forming were higher (P=0.03) when ghrelin was not be added to the IVM medium. We found that ghrelin negatively impacts CC viability and pronuclear formation.