Combined Effect of Retinoic Acid and Basic Fibroblast
Growth Factor on Maturation of Mouse Oocyte and
Subsequent Fertilization and Development
Many autocrine and paracrine elements that are produced within follicular niche have been the focus of
Materials and Methods
In this experimental study, germinal vesicle (GV) oocytes obtained from two-months-old
NMRI mice were randomly divided into control, sham and three experimental groups. The basic culture medium
was α-MEM supplemented with 10% fetal bovine serum (FBS), 50 mg/l streptomycin, 60 mg/l penicillin and 10 ng/
ml epidermal growth factors. Each of the experimental groups received one of the following treatments: RA (2 µM),
bFGF (20 ng/ml) or combination of RA and bFGF with the indicated concentrations. After 24 hours, capacitated spermatozoa were added to
As compared with the control group, the rate of maturation was significantly increased in the RA (P<0.001) and bFGF+RA (P<0.02) groups with 58 ± 10 and 57 ± 3.46, respectively. The rate of maturation was significant in the RA (P<0.02) and bFGF+RA (P<0.03) groups, in comparison with the bFGF group. The bFGF+RA group had higher rate (83 ± 1.52) of two-cells development, than control (33 ± 1, P<0.001).
Our findings showed beneficial effects of 2 µM RA and 20 ng/ml bFGF combination on mouse oocyte IVM.