Multiplex-Polymerase Chain Reaction for Detecting
Microdeletions in The Azoospermia Factor Region of
Y Chromosome in Iranian Couples with Non-Obstructive
Infertility and Recurrent Pregnancy Loss
Approximately 15% of couples are infertile with the male factor explaining approximately 50% of the
cases. One of the main genetic factors playing a role in male infertility is Y chromosomal microdeletions within the
proximal long arm of the Y chromosome (Yq11), named the azoospermia factor
Materials and Methods
In this is a case-control study, genomic DNA was extracted from 80 male samples including 40 non-obstructive infertile men, 20 males from couples with RPL and 20 fertile males as controls. Multiplex polymerase chain reaction was used to amplify 19 sequence tagged sites (STS) to detect AZF microdeletions. Differences between the case and control groups were evaluated by two-tailed unpaired t test. P<0.05 were considered statistically significant.
Only one subject was detected to have Y chromosome microdeletions in
Performing Testing for Y chromosome microdeletions in men with non-obstructive infertility and couples with RPL remains inconclusive in this study.