Current Issue

Volume 11, Number 4, Jan-Mar 2018 Pages: 270-278

N-Acetylcysteine Compared to Metformin, Improves The Expression Profile of Growth Differentiation Factor-9 and Receptor Tyrosine Kinase c-Kit in The Oocytes of Patients with Polycystic Ovarian Syndrome


Ebrahim Cheraghi, Ph.D, 1, Malek Soleimani Mehranjani, Ph.D, 2, *, Seyed Mohammad Ali Shariatzadeh, Ph.D, 2, Mohammad Hossein Nasr Esfahani, Ph.D, 3, Behrang Alani, Ph.D, 4,
Department of Biology, Faculty of Sciences, University of Qom, Qom, Iran
Department of Biology, Faculty of Science, Arak University, Arak, Iran
Department of Reproductive Biotechnology, Reproductive Biomedicine Research Center, Royan Institute, ACECR, Isfahan, Iran
Department of Applied Cell Science, Faculty of Medicine, Kashan University of Medical Sciences, Kashan, Iran
*Corresponding Address: P.O.Box: 381-5688138 Department of Biology Faculty of Science Arak University Sardasht Arak Iran Email: M-soleimani@araku.ac.ir

Abstract

Background

Paracrine disruption of growth factors in women with polycystic ovarian syndrome (PCOS) results in production of low quality oocyte, especially following ovulation induction. The aim of this study was to investigate the effects of metformin (MET), N-acetylcysteine (NAC) and their combination on the hormonal levels and expres- sion profile of GDF-9, BMP-15 and c-kit, as hallmarks of oocyte quality, in PCOS patients.

Materials and Methods

This prospective randomized, double-blind, placebo controlled trial aims to study the effects of MET, NAC and their combination (MET+NAC) on expression of GDF-9, BMP-15 and c-kit mRNA in oocytes [10 at the germinal vesicle (GV) stage, 10 at the MI stage, and 10 at the MII stage from per group] derived following ovulation induction in PCOS. Treatment was carried out for six weeks, starting on the third day of previous cycle until oocyte aspiration. The expression of GDF9, BMP15 and c-kit were determined by quantitative real time polymerase chain reaction (RT-qPCR) and western blot analysis. Data were analyzed with one-way ANOVA.

Results

The follicular fluid (FF) level of c-kit protein significantly decreased in the NAC group compared to the other groups. Significant correlations were observed between the FF soluble c-kit protein with FF volume, androstenedione and estradiol. The GDF-9 expression in unfertilized mature oocytes were significantly higher in the NAC group com- pared to the other groups (P<0.001). Similar difference was not observed between the MET, NAC+MET and control groups. The c-kit expression in unfertilized mature oocytes were significantly lower in the NAC group compared to the other groups (P<0.001). Similar difference was not observed between the MET, NAC+MET and control groups (Registration number: IRCT201204159476N1).

Conclusion

We concluded that NAC can improve the quality of oocytes in PCOS.