Comparison of The Effects of Vitrification on Gene Expression
of Mature Mouse Oocytes Using Cryotop and Open Pulled Straw
Oocyte cryopreservation is an essential part of the assisted reproductive technology (ART), which was recently introduced into clinical practice. This study aimed to evaluate the effects of two vitrification systems-Cryotop and Open Pulled Straw (OPS)-on mature oocytes gene expressions.
Materials and Methods
In this experimental study, the survival rate of metaphase II (MII) mouse oocytes were
assessed after cryopreservation by vitrification via i. OPS or ii. Cryotop. Then we compared the fertilization rate of
oocytes produced via these two methods. In the second experiment, we determined the effects of the two vitrification
methods on the expression of
Our results indicated that Cryotop vitrification increases both cooling and warming rates, but both Cryo- top and OPS techniques have the same effect on the mouse oocytes after vitrification.