Comparative Stepwise Pattern of Reactive Oxygen
Species Production during In Vitro Development of
Fertilized and Nuclear Transferred Goat Embryos
A unique feature of embryo metabolism is production of reactive oxygen
species (ROS). It is well established that during
Materials and Methods
In this experimental study, zona-free oocytes, SCNT and IVF
embryos at different stages of
The relative level of ROS compared to metaphase-II (MII) oocytes insignificantly decrease during early stages post embryo reconstitution and regained its value by 8-cell and morula stage and, significantly increase compared to MII oocytes by blastocyst stage.
The pattern of ROS change in SCNT embryos is similar to zona free IVF derived embryos, except it decrease from two cell stage and regain its value at morula stage. The sudden rise in ROS at blastocyst stage, further emphasizes the special need of IVF and SCNT derived embryos during this stage of development.