Past Issue

Volume 10, Number 3, Oct-Dec 2016, Pages: 297-302

Cord Blood Karyotyping: A Safe and Non-Invasive Method for Postnatal Testing of Assisted Reproductive Technology Children

Shabnam Zarei Moradi, M.Sc., 1, Najmehsadat Masoudi, M.Sc., 1, Anahita Mohseni Meybodi, Ph.D., 1, Khadijeh Anisi Hemaseh, M.Sc., 1, Ramin Mozafari Kermani, M.D., 2, Abolhasan Shahzadeh Fazeli, M.D., 1, 2, Hamid Gourabi, Ph.D., 1, *,
Department of Genetics , Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran
Child Health and Development Research Center, Iran Medical Science Branch of ACECR, Tehran, Iran
* Corresponding Address: P.O.Box: 16635-148 Department of Genetics Reproductive Biomedicine Research Center Royan Institute for Reproductive Biomedicine ACECRTehranIran Email:



To verify the hypothesis that the incidence of chromosomal abnormalities increases in babies conceived by different assisted reproduction procedures. The availability of the umbilical cord blood encouraged us to study this hypothesis via this method.

Materials and Methods:

This is a descriptive study, umbilical cord blood samples of assisted reproductive technology (ART) children were analyzed with standard cytogenetic techniques (G banding). Karyotyping was possible in 109 cases.


The number of abnormal cases was four (3.7%), among which, three cases (2.8%) were inherited and only 1 case (0.9%) was a de novo translocation. In total, the incidence of de novo chromosomal abnormalities was in the range observed in all live births in the general population (0.7-1%).


No significant difference in the incidence of chromosomal abnormality was found between ART and naturally conceived babies. To date, several studies have examined the medical and developmental outcome of ART children and still have not reached a definite conclusion. Genetic counseling is recommended as an integral part of planning of treatment strategies for couples wishing to undergo ART.