Current Issue

Volume 11, Number 4, Jan-Mar 2018 Pages: 247-252

Gene Polymorphism of Matrix Metalloproteinase 9 in Asthenozoospermic Male Subjects


Sina Mohagheghi, M.Sc., 1, Iraj Khodadadi, Ph.D., 2, Manoochehr Karami, Ph.D., 3, Iraj Amiri, Ph.D., 4, Heidar Tavilani, Ph.D., 5, *,
Student Research Center, Hamadan University of Medical Sciences, Hamadan, Iran
Department of Biochemistry, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
Modeling of Non-Communicable Diseases Research Center, Department of Biostatistics and Epidemiology, School of Public Health, Hamadan University of Medical Sciences, Hamadan, Iran
Endometrium and Endometriosis Research Center, Hamadan University of Medical Sciences, Hamadan, Iran
Urology and Nephrology Research Center, Hamadan University of Medical Sciences, Hamadan, Iran
*Corresponding Address: P.O.Box: 6517838678 Urology and Nephrology Research Center Hamadan University of Medical Sciences Shaheed Fahmideh Ave. Hamadan Iran Email: tavilani@gmail.com

Abstract

Background:

Matrix metalloproteinase (MMPs) play important roles in the structural and functional properties of reproductive organs. The aim of this study is to determine the prevalence of C-1562T MMP-9 (rs3918242) gene polymorphism in fertile and infertile men. In addition, we aim to determine the association between C-1562T MMP-9 and G-1575A MMP-2 gene polymorphisms.

Materials and Methods:

A total of 400 subjects, including 200 fertile and 200 infertile men, were recruited for this casecontrol study. The allele frequencies and genotype distributions of single nucleotide polymorphism in the promoter regions of MMP-9 (C-1562T) were determined using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. The chi-square (χ2) test was used to assess the distribution of genotype frequencies.

Results:

There were no significant differences found in the genotype distributions or allele frequencies between fertile and infertile men for the C-1562T MMP-9 gene polymorphism. The percent of immotile sperm in infertile men with the CC and CT genotypes of C-1562T MMP-9 gene polymorphism significantly differed compared with that of subjects with the TT genotype. The frequency of CC/GA-combined genotypes of C-1562T MMP-9 and G-1575A MMP-2 gene polymorphisms significantly differed in fertile and infertile men (P=0.031).

Conclusion:

Our results suggest that genetic polymorphisms in MMP may impact male fertility.