Past Issue

Volume 10, Number 2, Jul-Sep 2016, Pages: 215-222

Human Sperm Quality and Metal Toxicants: Protective Effects of some Flavonoids on Male Reproductive Function


Mostafa Jamalan, Ph.D, 1, Mohammad Ali Ghaffari, Ph.D, 2, 3, *, Pooneh Hoseinzadeh, M.Sc, 2, Mahmoud Hashemitabar, Ph.D, 2, 4, Majid Zeinali, Ph.D, 5,
Abadan School of Medical Sciences, Abadan, Iran
Cellular and Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
Department of Biochemistry, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
Department of Anatomical Sciences, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
Biotechnology Research Center, Research Institute of Petroleum Industry (RIPI), Tehran, Iran
*Corresponding Address: P.O.Box: 159 Cellular and Molecular Research Center Ahvaz Jundishapur University of Medical Sciences Ahvaz Iran Email:ghaffari@ajums.ac.ir

Abstract

Background

Metals can cause male infertility through affection of spermatogenesis and sperm quality. Strong evidences confirm that male infertility in metal-exposed humans is mediated via various mechanisms such as production of reactive oxygen species (ROS). Flavonoids have antioxidant and metal chelating properties which make them suitable candidates for neutralizing adverse effects of metals on semen quality. In the current study, we have evaluated the effects of five types of flavonoids (rutin, naringin, kaempferol, quercetin, and catechin) on recovery of sperm motility and prevention of membrane oxidative damage from aluminum chloride (AlCl3), cadmium chloride (CdCl2), and lead chloride (PbCl4).

Materials and Methods

In this experimental study, motility and lipid peroxidation of metalexposed sperm was investigated in the presence of different concentrations of five kinds of flavonoids. Malondialdehyde (MDA) production was assessed as a lipid peroxidation marker.

Results

Aluminum chloride (AlCl3), cadmium chloride (CdCl2), and lead chloride (PbCl4) diminished sperm motility. Treatment of metal-exposed sperm with rutin, naringin, and kaempferol attenuated the negative effects of the metals on sperm motility. Quercetin and catechin decreased the motility of metal-exposed sperm.

Conclusion

Based on the MDA production results, only AlCl3 significantly induced lipid peroxidation. Treatment with rutin, naringin, and kaempferol significantly decreased MDA production.