Past Issue

Volume 10, Number 2, Jul-Sep 2016, Pages: 208-214

Thyroxin Is Useful to Improve Sperm Motility


Gabriela Ruth Mendeluk, Ph.D, 1, *, Mónica Rosales, M.D, 2,
Laboratory of Male Fertility, Hospital de Clínicas José de San Martín, Faculty of Pharmacy and Biochemistry, University of Buenos Aires, Buenos Aires, Argentina
Laboratory of Endocrinology, Hospital de Clínicas José de San Martín, Faculty of Pharmacy and Biochemistry, University of Buenos Aires, Buenos Aires, Argentina
*Corresponding Address: Laboratory of Male Fertility Hospital de Clínicas José de San Martín Faculty of Pharmacy and Biochemis- try University of Buenos Aires Av. Córdoba 2351 (1120) Buenos Aires Argentina Email:gmendeluk@ffyb.uba.ar

Abstract

Background

The aim of this study was to evaluate the non-genomic action of thyroxin on sperm kinetic and its probable use to improve sperm recovery after applying an en- richment method like “swim-up” in comparison with the available one, pentoxifylline.

Materials and Methods

This is an experimental study. A total of 50 patients were re- cruited, followed by infertility consultation. Conventional sperm assays were performed according to World Health Organization criteria-2010 (WHO-2010). A Computer Aided Semen Analysis System was employed to assess kinetic parameters and concentrations. Number of the motile sperm recovered after preparation technique was calculated.

Results

Addition of T4 (0.002 µg/ml) to semen samples increased hypermotility at 20 minutes (control: 14.18 ± 5.1% vs. 17.66 ± 8.88%, P<0.03, data expressed as mean ± SD) and remained unchanged after 40 minutes. Significant differences were found in the motile sperm recovered after swim-up (control: 8.93×106 ± 9.52× 06vs. 17.20×106 ± 21.16×106, P<0.03), achieving all of the tested samples a desirable threshold value for artificial insemination outcome, while adding pentoxifylline increased the number of recovered sperm after swim-up in 60% of the studied cases. No synergism between two treatments could be determined.

Conclusion

We propose a new physiological tool to artificially improve insemination. The discussion opens windows to investigate unknown pathways involved in sperm ca- pacitation and gives innovative arguments to better understand infertility mechanisms.