Current Issue

Volume 11, Number 2, Jul-Sep 2017 Pages: 85-92

Vitrification of Human Germinal Vesicle Oocytes: before or after In Vitro Maturation?


Evangelia Kasapi, B.Sc, 1, 2, Byron Asimakopoulos, Ph.D, 2, *, Katerina Chatzimeletiou, Ph.D, 3, Stamatios Petousis, M.D., M.Sc., 1, Yannis Panagiotidis, Ph.D, 1, Nikos Prapas, M.D., Ph.D., 1, Nikos Nikolettos, M.D., Ph.D., 4,
Iakentro Fertility Centre, IVF Laboratory, Thessaloniki, Greece
Democritus University of Thrace, Laboratory of Physiology, Faculty of Medicine, School of Health Sciences, Alexandroupolis, Greece
Aristotle University of Thessaloniki, Medical School, 1st Department of Obstetrics and Gynaecology, Papageorgiou General Hospital, Thessaloniki, Greece
Embryokosmogenesis, IVF Laboratory, Alexandroupolis, Greece
*Corresponding Address: Democritus University of Thrace Laboratory of Physiology Faculty of Medicine School of Health Sciences Alexandroupolis Greece Email:basima@med.duth.gr

Abstract

Background

The use of immature oocytes derived from stimulated cycles could be of great importance, particularly for urgent fertility preservation cases. The current study aimed to determine whether in vitro maturation (IVM) was more successful before or after vitrification of these oocytes.

Materials and Methods

This prospective study was performed in a private in vitro fertilization (IVF) center. We collected 318 germinal vesicle (GV) oocytes from 104 stimulated oocyte donation cycles. Oocytes were divided into two groups according to whether vitrification was applied at the GV stage (group 1) or in vitro matured to the metaphase II (MII) stage and then vitrified (group 2). In the control group (group 3), oocytes were in vitro matured without vitrification. In all three groups, we assessed survival rate after warming, maturation rate, and MII-spindle/chromosome configurations. The chi-square test was used to compare rates between the three groups. Statistical significance was defined at P<0.05 and we used Bonferroni criterion to assess statistical significance regarding the various pairs of groups. The Statistical Package for the Social Sciences version 17.0 was used to perform statistical analysis.

Results

There was no significant difference in the survival rate after vitrification and warming of GV (93.5%) and MII oocytes (90.8%). A significantly higher maturation rate occurred when IVM was performed before vitrification (82.9%) compared to after vitrification (51%). There was no significant difference in the incidence of normal spindle/ chromosome configurations among warmed oocytes matured in vitro before (50.0%) or after (41.2%) vitrification. However, a higher incidence of normal spindle/chromosome configurations existed in the in vitro matured oocytes which were not subjected to vitrification (fresh oocytes, 77.9%).

Conclusion

In stimulated cycles, vitrification of in vitro matured MII oocytes rather than GV oocytes seems to be more efficient. This approach needs to be verified in nonstimulated fertility preservation cases.