Expression Profile of Developmentally Important Genes
in preand peri-Implantation Goat Embryos
Produced In Vitro
The first two authors equally contributed to this manuscript.
Little is understood about the regulation of gene expression during early
goat embryo development. This study investigated the expression profile of 19 genes,
known to be critical for early embryo development in mouse and human, at five different
stages of goat
Materials and Methods:
In this experimental study, stage-specific profiling using real time-quantitative polymerase chain reaction (RT-qPCR) revealed robust and dynamic patterns of stage-specific gene activity that fall into four major clusters depending on their respective mRNA profiles.
The gradual pattern of reduction in the maternally stored transcripts without renewal thereafter (cluster-1:
Evolutionary comparison revealed that the selected subset of genes has been rewired in goat and human/goat similarity is greater than the mouse/goat or bovine/goat similarities. The developed profiles provide a resource for comprehensive understanding of goat preimplantation development and pluripotent stem cell engineering as well.