Effect of In Vitro Maturation Technique and Alpha Lipoic
Acid Supplementation on Oocyte Maturation Rate:
Focus on Oxidative Status of Oocytes
Therapeutic potential of
Materials and Methods
In this experimental study, mouse germinal vesicle (GV) oocytes
divided into cumulus denuded oocytes (DOs) and cumulus oocyte complexes (COCs) groups.
GVs were matured
Maturation rate of COCs was significantly higher than DOs in control group (P<0.05), while there was no significant difference between COCs and DOs when were pre-cultured with forskolin plus cilostamide. ROS and TAC levels was increased and decreased respectively in DOs after 18 hours while in COCs did not change at 18 hours and showed a significant increase and decrease respectively at 36 hours (P<0.05). ROS and TAC levels in the presence of ALA were significantly decreased and increased respectively after 36 hours (P<0.05) whereas, maturation rates of COCs and DOs were similar to their corresponding control groups.
ALA decreased ROS and increased TAC but could not affect maturation rate of both COCs and DOs in one or two step IVM manner.