Spontaneous abortion has been defined as two or more consecutive miscarriages at 20 weeks pregnancy. Vascular endothelial growth factor (VEGF) plays a main role in fetal and placental angiogenesis which secretes from different cells like endometrium and placenta.The objective of this study was to investigate of association of VEGFA gene polymorphisms and recurrent spontaneous abortion (RSA).
Materials and methods
In this project, a case-control study of 100 women with RSA and 100 fertile controls was performed to evaluate four VEGF gene polymorphisms including + 936C/T (rs3025039) and -154G>A (rs1570360). The polymorphisms of VEGF gene were studied by using PCR-RFLP technique. Restriction enzymes consist of NlaIII and MnlI were used for digestion. Digestion products were visualized by polyacrylamide gel (12%PAGE).The results were analyzed in the two studied groups by using SPSS version 18, SNPAlyze 7 and GenAlex Ver. 6.4.
We found association between -154G/A (rs1570360,5´-UTR)[OR (95% CI)=0.01 (0.03-0.29); P<0.0001] heterozygous genotype (GA) and RSA. The VEGFA single nucleotide polymorphism (SNP) +936C/T (rs3025039, 3´UTR region) [OR (95% CI)= 0.73 (0.33-1.60); P<0.43] did not show association with RSA pathogenesis.The genetic variation of studied population was estimated by Nei’s genetic diversity and Shannon index. Cluster analysis including Neighbor Joining and K-means clustering as well as analysis of molecular variance (AMOVA) supported genetic differentiation of women with RSA and controls.
Allelic polymorphisms in common VEGF SNPs was associated with RSA samples and haplotypes with at least one minor allele showed association with RSA pathogenesis.