Male infertility is a multifactorial syndrome encompassing a wide variety of disorders. In more than half of infertile men, the cause of their infertility is unknown (idiopathic) and could be congenital or acquired. OGG1 is a bifunctional glycosylaseii, in that it has both AP lyase and DNA glycosylase activity and one of its functions is to excise and remove 8-oxo-dGuo from DNA. Among the common single nucleotide polymorphisms (SNPs) of the OGG1 gene, one located in exon 7, resulting in an amino acid substitution of serine (Ser) with cysteine (Cys) at codon 326 (Ser326Cys, rs1052133), has been demonstrated to affect OGG1 function. Functional studies have revealed that 326Cys allele of OGG1 was associated with the reduced repair capacity toward oxidized DNA lesions. The aim of this study was to elucidate the relationship between OGG1 Ser326Cys polymorphism and Idiopathic male infertility.
Materials and methods
Frothy eight men with idiopathic infertility and 50 healthy men were included in this study. DNA was extracted from 1 ml of peripheral blood samples. Ser326Cys polymorphism was determined using PCR-RFLP method. Statistical analysis was performed using the MedCalc program.
In the control group, the distribution of genotypes was as follows: 88% were Ser/Ser, 10% were Ser/Cys and 2% were Cys/Cys. For the infertile group, the distributions were 83% Ser/Ser, 13% Ser/Cys and 4% Cys/Cys.
The results of this study suggested that the OGG1 Ser326Cys polymorphism may not associated with male infertility. However, additional studies are needed to validate our findings.