P-118: Triplet Nucleotide Repeats Expansion (CAG and GGN) of Androgen Receptor Gene in Infertile Patients with Abnormal Spermogram
The infertility has recently been estimated to affect approximately 9% of couples worldwide. Androgens and a functional androgen receptor (AR) are essential for normal development of the male gender, and for maintenance of spermatogenesis throughout the life. Two polymorphic trinucleotide repeats, CAG and GGN, encoding for the amino acids glutamine and glycine, respectively are located on the longest exon of AR gene. The effect of these trinucleotide repeats expansions on AR actions and male infertility is still under investigation as an interesting question. This study determines to answer this question in Iranian population.\"ho
Materials and methods
Therefore 80 infertile patients and 40 control fertile subjects were elected. The infertile group was divided into two subgroups according to the sperm count (40 asthenospermic men and 20 severoligospermic men). After DNA extraction from peripheral blood and amplifying the AR gene via polymerase chain reaction (PCR), number of CAG and (GGN)n in each group were determined by sequencing method.n��
Median lengths CAG for the asthenospermic men and the control group respectively were 24.12 and 21.8. Statistical analysis of the CAG repeats shows significant difference (P=0.0) between the asthenospermic and the control group. Nonsignificant difference was elicited between CAG repeat in the severoligospermic group and the control group (mean lengths 21.4 and 21.8, P=0.96). AR-GGN repeats have demonstrated nonsignificant difference in two asthenospermic and severoligospermic in compared with the control group.
This evidence suggests AR with long CAG stretches can display lower activity than the AR of median length. Reduced AR activity causes defect in sperm count and motility. In this study Association between GGN repeats and AR actins and sperm production was not conformed and needs more research.��