P-116: Absence of JMJD1A, A Testis- Specific Histone Demethylase, in Tissue Samples of TESE Negative Men
During mammalian spermatogenesis unique and dynamic epigenetic events occur leading to chromatin condensation. Through these events, histone demethylases such as JMJD1A play important roles in compaction of sperm chromatin, due to regulation of histone methylation dynamics and alteration of chromatin structure. As �histone methylation� is one of the best-characterized modifications in the study of germ cell development, evaluation of presence/ absence of JMJD1A protein in impaired spermiogenesis was aimed in this study.
Materials and methods
For this respect, based on spermogram and presence/absence of sperm in testicular sperm extraction (TESE) process, testis tissue samples of infertile men referred to Royan Institute were collected as two groups of TESE negative (n=9) and TESE positive (n=3), respectively. Immunohistochemical analysis of paraffin embedded tissue samples was performed qualitatively, using anti-JMJD1A antibody to elucidate presence/ absence of JMJD1A in nucleus of germ cells.
Immunohistochemical analysis data showed absence of JMJD1A protein in nucleus of germ cells in TESE negative group compared to TESE positive group.n��
It can be concluded that there is an obvious association between absence of histone demethylation as a chromatin condensing state with impairment of spermatogenesis and male infertility.