P-115: The Role of G22A Adenosine Deaminase 1 Gene Polymorphism and The Activities of ADA Isoenzymes in Fertile and Infertile Men
Some studies have revealed enzymatic and non-enzymatic roles of adenosine deaminase (ADA) in male reproductive system such as regulating adenylate cyclase activity by decreasing adenosine concentration, helping to interaction between prostasomes and spermatozoa.
Materials and methods
frequency distribution of ADA1 G22A alleles and genotypes in 200 fertile and 200 infertile men. The polymerase chain reaction– restriction fragment length polymorphism (PCR-RFLP) technique was used for determining of ADA1 G22A variants. In addition, ADA isoenzymes activities (ADA1 and ADA2) were measured using Giusti and Galanti colorimetric method.
The frequency of GG genotype was significantly higher and GA genotype was lower in infertile males compared with fertile men (P=0.048 and P=0.045 respectively). However, there was not any noticeable difference in allele distribution between groups (P>0.05). Based on logistic regression analysis, the GA genotype has a protective role and can decrease the risk of male infertility 1.7 times (P=0.046). There were significantly higher activities of ADAT and its isoenzymes in infertile males compared with fertile men (P<0.05). Also, ADA1 activity with GG genotype was higher than GA carriers in all population (P=0.001).
Our results revealed that activity of ADA isoenzymes and distribution of ADA1 G22A genotypes were different among fertile and infertile men and more likely the GA genotype which had lower ADA1 activity and was higher in fertile men is a protective factor against infertility. Moreover, activity of ADATotal, ADA1 and ADA2 enzymes were significantly higher in infertile men compared with fertile subjects which can indicate importance of this enzyme in male fertility.