Varicocele is dilatation of the veins of the pampiniform plexus within the spermatic cord and is one of the amendable causes of male infertility. Many studies have revealed increase of oxidative stress in serum, semen and testicular tissues of patients with varicocele which may cause DNA damage. HSP70 are a family of proteins that can be expressed in response to a variety of stress, including reactive oxygen species (ROS) and play an important role in the maintenance of cellular integrity. The aim of this study was the evaluation of HSP70-hom polymorphism in infertile men with varicocele.
Materials and methods
The total number of 86 men (37 patients with varicocele and 49 normal controls) was enrolled into the study. Genomic DNA was isolated from 1 ml peripheral blood. Genotyping was performed by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Statistical analysis was performed using the MedCalc program (Version 12.1).
The frequency of the TT, TC and CC genotype of HSP70-hom polymorphism in varicocele patients were 75.5, 20.41 and 4.1%, respectively, while in controls were 72.9, 8.1 and 18.9%, respectively.
It is suggested the HSP70-hom may affect the susceptibility to varicocele in the studied population. While the randomized multicenter studies with greater, sample size are still needed to clarify our results.