P-105: Genetic Variation of Kinase Insert Domain-Containing Receptor Gene and Its Association with Recurrent Spontaneous Abortion
Recurrent spontaneous abortion has been defined two or more consecutive miscarriages at 20 weeks pregnancy and one of diseases that can lead to physical, psychological and economical for the individual problems. Recently number of polymorphisms in several genes was examined for association analyses in pregnant women which related to endanger the life of the fetus. In present study we investigated allele frequency of SNP in Kinase insert Domain Receptor (KDR) gene in women with recurrent miscarriage.
Materials and methods
One hundred women with recurrent spontaneous abortion of the fetus with at least 2 or more miscarriages before 20 weeks and without anatomical problems of the uterus, cytogenetic, hormonal problems as patients and 100 women with no history of abortion and with having had successful birth were chosen as controls. For polymorphism analysis of functional SNP rs1870377, PCR-RFLP was performed by using the restriction enzymes AluI and digested products were visualized on 12% acrylamide gel respectively. The differences between allele frequency in two grouped were calculated by chi square test with P value<0.05. The results were analyzed in the both groups by using SPSS version 18, SNPAlyze 7 and GenAlex Ver. 6.4,.
The estimated risk of subjects with one or two copies of risk alleles in different inheritance models showed no significant differences for crude odd ratio in 95% confidence interval (OR = 1.62, 1.05 for rs1870377 respectively. while, rs1870377 with X2 =3.249, P=0.08 showed slightly significant difference (P<0.1).K means clustering showed k = 8 as the best fit for the optimal number of genetic subgroups in our studied materials similar to NJ cluster analysis.
Inconsistent results in different ethnic groups with different allele frequencies among RSA patients and controls may be involving two main factors including, ethnic variation and sample size in these studies.