Past Issue

Volume 9, Supplement 1, Summer 2015 (Presented at 16th Congress on Reproductive Biomedicine and 10th Royan Nursing and Midwifery Seminar) Pages: 83-83

P-95: Seasonal Changes in Hypothalamic Gonadotropin-Inhibitory Hormone Expression in Alectoris Chukar


Background
Gonadotropin-inhibitory hormone (GnIH), initially discovered in birds as a hypothalamic neuropeptide, inhibits the synthesis and release of gonadotropins via affecting GnRH neurons and gonadotropes. Therefore, it could be a key neuropeptide in regulating seasonal breeding in birds. The aim of the present study was to investigate the expression of GnIH in the hypothalamus of male and female chukar partridges during the breeding and non-breeding seasons.
Materials and methods
In breeding (May) and nonbreeding (January) seasons, the brains of male (n=10) and female (n=10) sexually mature chukar partridges were removed following fixation. Sections (30 μm) were prepared from the entire hypothalamus and stained immunohistochemically (GnIH antibody was provided by Prof. K. Tsutsui).
Results
GnIH-immunoreactive (-IR) neurons were primarily found in PVN nucleus and few positive neurons detected in DMN nucleus. The number of GnIH-ir neurons were significantly lower in the breeding season than non-breeding season in both male and female partridges (P<0.05).
Conclusion
The results showed that GnIH neurons may play part in regulating the seasonal breeding in the chukar partridge.