P-91: Vitamin E and Selenium Supplementation Affects Aldehyde Oxidase, Xanthine Dehydrogenase/Oxidase Activities In Diabetic Rat Ovaries
Any factor like diabetes that changes the oxidant/antioxidant balance in favor of oxidants could possibly disrupt the physiologic function of ovaries. So, we have investigated the effect of vitamin E and Se supplements on antioxidant defense in ovaries of diabetic rats with focus on Xanthine dehydrogenase/oxidase (XDH/XO) and Aldehyde oxidase (AO) activities.
Materials and methods
This study was conducted on three groups: control C; STZ-induced diabetic, DM; and diabetic rats with orally administered vitamin E and selenium, DM+S. After 4 weeks treatment, plasma and ovarian levels of Malon-dialdehyde (MDA), total antioxidant status (TAS) and also, ovarian activities of Superoxide dismutase (SOD), Glutathione peroxidase (GPX), AO and XO/XDH were evaluated.
Ovarian GPX activity and TAS level in DM group were lower than DM+S and control (P<0.001). Although the supplementation significantly decreased MDA level and activities of XO, XDH and AO in ovaries (P<0.05) but they were significantly higher than those in C group (P<0.05). In group C, there were positive correlations between XO activity with ovarian TAS levels and also between AO with GPX activities (r=0.83, p=0.01; r=0.77, P=0.02 respectively). Furthermore, a significant positive correlation was seen between XO and GPX activities in DM+S group (r=0.85, P=0.007). There was a statistically positive correlation between plasma and ovary levels of MDA in control group (r=0.787, P=0.02).
Our study showed that in ovarian tissue of diabetic rat the activity of GPX and TAS levels were decreased and the level of MDA and AO, XDH and XO activities were increased. It also became clear that vitamin E and Se supplementation in diabetic rats could increase the activity of antioxidant enzymes and decrease activity of oxidant enzymes to improve the situation.