Sample preparation is the first step in the evaluation of tissue samples. In this research tried to using two types of formalin and Bouin fixative ovarian tissue morphogenesis be evaluated practical.
Materials and methods
Ovaries of 6-8 week-old Naval Medical Research Institute female mice were randomly divided into 3 groups: Ι. Ovaries that immediately fixed in Bouin’s fixative; II. Ovaries that immediately fixed in Formalin’s fixative; and III. Ovaries that in the first for 24 hours at Bouin’s fixative and were subsequently fixed in Formalin for 24 hours. The samples were serially sectioned and stained either with H&E or immunohistochemistry kit of pro-caspase-3.
Morphologically healthy primary, preantral and antral follicles showed significant reductions in II group compared to Ι and III groups. The difference in the number of primordial, primary, preantral and antral healthy and dead follicles between the Ι and III groups was not significant. In comparison with healthy follicles, there were significantly more dead follicles in the II group than the other groups. The apoptotic follicles increased significantly in III group compared to Ι group. No results were obtained from formalin-fixed specimens immunohistochemistry study.
The results showed that formalin solution is not appropriate fixative for immunohistochemistry studies. According to the results of this research, it can be stated that, for fixing ovarian tissue Bouin’s fixative is a more appropriate solution.