Past Issue

Volume 9, Supplement 1, Summer 2015 (Presented at 16th Congress on Reproductive Biomedicine and 10th Royan Nursing and Midwifery Seminar) Pages: 78-78

P-83: Development of Mouse Preantral Follicles in Fibrin-Alginate Matrix during In Vitro Culture


Background
This research was conducted to assess preantral follicle development in fibrin alginate matrix following ovarian tissue vitrification
Materials and methods
Ovaries of 13-day-old NMRI female mice were removed and placed in control and vitrification groups. Vitrification group ovaries were transferred in media containing ethylene glycol, dimethyl sulphoxide, and sucrose then were plunged in LN2 by acupuncture needle. Medium sized preantral follicles of fresh control and vitrified-warmed ovaries were mechanically isolated and cultured in fibrin-alginate matrix for 12 days. Finally, survival and growth rate and also quantitative oocyte maturation genes (Gdf9 and Bmp15) expression of follicles were evaluated on 1st and 12th culture days
Results
Although higher follicle survival rate was shown in control group rather than vitrification one, follicle diameter was being increased until the last culture day in both groups. Quantitative oocyte maturation genes expression evaluation did not reveal any significant difference between control and vitrification groups on first and last days of culture, but they were significantly decreased on 12th culture day compared to the first in both groups
Conclusion
According to the observed similarities in both groups, it seems that fibrin-alginate could be an appropriate matrix for mouse preantral follicle development and in spite of decreased follicle survival rate in vitrification group, applied vitrification method did not affect developmental competence of ovarian follicles