Past Issue

Volume 9, Supplement 1, Summer 2015 (Presented at 16th Congress on Reproductive Biomedicine and 10th Royan Nursing and Midwifery Seminar) Pages: 68-69

P-61: Effect of 830 nm Diode LASER Irradiation on Human Sperm Motility


Background
Sperm motility is known as an effective parameter in male fertility and it depends on energy consumption. Low level LASER irradiation could increases energy supply to the cell by producing of adenosine triphosphate (ATP). The purpose of this study is to evaluate how the low level LASER irradiation affects the human sperm motility.
Materials and methods
Fresh human semen specimens of asthenospermic patients were divided into 4 equal portions and irradiated by 830nm GaAlAs LASER irradiation with varying doses as: 0 (control), 4, 6 and 10 J/cm2. At the times of 0, 30, 45 and 60 minutes following irradiation, sperm motilities are assessed by means of Computer-Aided Sperm Analysis (CASA) in all samples. Two additional tests (HOS and SCD tests) also performed on the control and high irradiated groups as well.
Results
Sperm motility of the control groups significantly decreased after 30, 45 and 60 min of irradiation, while those of irradiated groups remained constant or slightly increased by passing of time. Significant increases have been observed in doses of 4 and 6 J/cm2 at the times of 60 and 45 min, respectively. SCD test also revealed a significant difference.
Conclusion
Our results showed that irradiating human sperms with low level 830nm diode LASER can improve their progressive motility depending on both LASER density and post-exposure time.