Past Issue

Volume 9, Supplement 1, Summer 2015 (Presented at 16th Congress on Reproductive Biomedicine and 10th Royan Nursing and Midwifery Seminar) Pages: 68-68

P-60: Survival Assessment of Mouse In Vitro Embryos after Exposure to Cell Phone Radiation


Background
Using cellular phone among adults, teenagers and children has rapidly increased all over the world. Also, the concern on the possible health hazards of Electromagnetic Fields (EMF) induced from cell phones on reproduction has been growing in many countries. The aim was to assess the morphological parameters, survival rate and development of mouse in-vitro embryos obtained by natural breeding as consequences of exposure to the cell phone radiation (talk mode) during incubation.
Materials and methods
For control and experimental groups, we used a total of 40 (20 females and 20 males), 2-3 months old BALB/c mice. In the morning after mating, the ovary burses were surgically removed and the oocytes were dissected out from the ampulla region and divided into two groups. They were cultured for 5 days in vitro, which one of them was exposed to RF radiation (30 min/day). Also, PI and H33342 immunostaining was performed for identification of viable blastocysts
Results
There was not significant change in the rate of embryo survival to the blastocyst stage in the experimental group after exposure to (900–1800 MHz) of EMF which emitted from cell phone; compared with control cultures (60.6% vs. 69.3%, P=0.15). Also, the loss of cell viability was found by significant increase in the percentage of necrotic and/or dead cells within an exposure blastocyst. (P=0.002)
Conclusion
The normal embryonic development up to the blastocyst stage indicates that EMF-exposure commonly did not have direct adverse effect on the early embryo development in mice. But, it may cause loss of cell viability and changes in membrane permeability and late apoptotic.