P-50: Evolution of Bovine Embryos Cleavage in Co-Cultuer Mesenchyme Stem Cells (MSCs) Deried from Adipose Tissues
Mesenchyme stem cells (MSCs) are one of the widely used sources in tissue engineering and efficacy of cultural environment. In this survey, rat stem cells are used as coculture. Owing to main obstacle of embryo culture in the lab medium, the survival of embryo in lab is low in comparison with that of normal conditions. The purpose of this research is the survey of fertilization and culture of bovine embryos in mesenchymal stem cells derived from adipose tissue (ADMCs).
Materials and methods
Isolated ADMCs were cultured on 4-well dishes. Three days after cell culture, the bovine immature oocytes were transferred on this mono layer system and the in vitro matured oocytes were fertilized by sperms in another MSCs free environment subsequently. Then, in vitro fertilized embryos were co-cultured with the same mono layer of ADMCs for seven days. During oocyte and embryo coculture, the rates of maturation and cleavage were assessed.
This study reveals that co-culture of embryos with mesenchymal stem cells could increase the survival and cleavage of embryos significantly (P<0.05). The maturation rate of bovine co-cultured oocytes (82%) in comparison with Abstracts of The 16th Royan International Congress on Reproductive Biomedicine 65 Int J Fertil Steril, Vol 9, Suppl 1, Summer 2015 that of control group (78%) has increased significantly. Also, the rate of blastocyst formation in co-cultured embryos (33%) was analytically comparable with that of control group (26%).
What bolsters substantially the quality and development of embryo is co-culture systems.