P-49: Protective Effect of Grape Seed Extraction on Dexamethason Induced Spermatogenesis Defect in Mice
Exposure to glucocorticoids such as dexamethasone (Dex) leads to numerous changes in various biological systems including the reproductive system .The present study investigates the efficacy grape seeds , source of the most useful groups of plant flavonoids, phenolic procyanids, to attenuate Dex-induced spetmatogenic defects in the mouse testicles.
Materials and methods
A total of 32 male mice were divided into four groups (8 mice each). The first group served as control and received distill water only for 25 days. Mice of the second group received Dex at the dose of 7 mg/kg body weight by i.p. injection for 7 days. Mice of the third group received efficacy grape seeds extract at a dose of 100 ml/kg body weight. The fourth group received efficacy grape seeds extract at the dose of 100 mg/kg for 25 days and Dex at the same dose of the second group was injected during the last 7 (18 to 25) days. Statistical signiﬁcance was determined using one-way analysis of varianc (ANOVA)followedby Tukey- Kramer multiple comparison tests. A P value <0.05 denoted the presence of a statistically signiﬁcant difference.
Dex induced changed the parameters of spermatozoa and Testicular histopathology in comparison with the control group. The spermatozoa parameters were altered in Dex + grape seeds extract group in a lesser extent than Dex animals (P<0.05). Dex caused epithelial vacuolization, sloughing of germ cells, reduction of seminiferous tubule diameter, and significant maturation arrest (P<0.05). grape seeds + Dex treatments showed significantly prevented these histopathologic changes (P<0.05).
It is concluded that, grape seeds extract may improve the adverse effects of Dex in the mouse testicular tissue by inducing anti-oxidant mechanism.