P-40: The Effect of Methanolic Extract of Avocado (Persea Americana) Seed on Quality and Quantity Index of Sperm in Mice with Diabetes Type II
Type II diabetes is a metabolic disorder that can gradually cause damage to the function of many organs such as the gonads. It is believed that poor blood glucose control is the main cause of diabetic side effects. Avocado (Persea americana) is a native tree of Central and South America that has traditionally been used due to its antioxidant, antiinflammatory, anti-hyperglycemic, anti-hyperlipidemic and anti-hypertensive effect but there is no scientific information about effect of Avocado seed extract on sperm characteristics in diabetic patients.
Materials and methods
In this study, 40 adult male mice were divided into 8 groups including: control, diabetic, 3 sham and 3 treatment groups. Diabetes type II was induced by high fat diet and low dose of streptozotocin (STZ). One week after STZ injection, sham and treatment groups received 50, 100 and 150 mg/kg methanolic extract of Avocado seed for 40 consecutive days orally via gavage. Finally, animals were euthanized and the epididymis was removed and dissected in Ham's F10 solution, incubated at 37°C and sperm motility was evaluated. Sperm were counted using a hemocytometer. Smears were prepared from the suspension stained with eosin-nigrosin and examined for sperm abnormalities by light microscope.
Sperm analysis indicated that however extract did not change total number of sperm but increased quality of sperm in high dosage in sham group compared to control significantly. Results showed that Avocado can significantly reduce malformed, abnormal and dead sperm and increase sperm with grade A and B in treatment group compared to diabetic animals (P<0.05) and bring it to control group in a dose-dependent manner.
It can be concluded that administration of methanolic extract of Avocado seed is a suitable protective strategy for side effect of diabetes and can increase fertilization ability in the diabetic males.