P-39: Protective Effect of Silymarin, Celecoxib and Testosterone on Varicocele- Induced Damages in Testis of Rats
Varicocele (VCL) is characterized by a progressive disorder which is defined by tortuosity of the pampiniform plexus veins. Although elevated oxidative stress, diminished endocrine status and severe inflammation are considered as main reasons for VCL-induced apoptosis, the role of estrogen receptors, testicular aromatization and proto-oncogenes under these conditions are remained unknown. Present study was done to uncover the therapeutic effect of silymarin (SMN, potent antioxidant), Celecoxib (CCB, COXII-inhibitor) and testosterone co-administration on mentioned parameters.
Materials and methods
Forty two mature male Wistar rats (2 months old) were randomly divided into 7 groups (N=6) as control-sham and experimental. VCL-induced animals in control-sham group received no chemical. The animals in experimental groups subdivided into 6 groups as; SMN alone-received (100 mg/kg), CCB alone-received (10 mg/kg), testosterone alone-received (40 μg/kg), SMN + CCBreceived, SMN + testosterone-received, CCB + testosteronereceived and SMN + testosterone+CCB-received groups. All chemicals were administrated orally, for 60 days. After 60 days testes were dissected out. The ERα, ERβ, p53 and bcl-2 expression was evaluated by using immunohistochemical and RT-PCR techniques. The mRNA level of cytochrome CYP19 was assessed by using RT-PCR.
Co-administration of SMN+CCB+testosterone significantly (P<0.05) up-regulated VCL-reduced ERα, bcl-2 (both at mRNA and protein levels) and CYP19 expression, and down-regulated VCL-increased ERβ, p53 expression. Simultaneous administration of SMN+CCB+testosterone exerted remarkably better results compared with alone forms of administration.
Our data suggested that co-administrating antioxidant, anti-inflammatory and exogenous testosterone promotes the endocrine statuses of the testis by ameliorating aromatization, elevating ERs and bcl-2 expression, and finally