The aim of this study is to examine the effects of cranberry extract prescription in sensitive days of pregnancy on development and histomorphometric changes in liver of mouse embryo.
Materials and methods
A total of 24 pregnant mice were divided into equal 4 groups. The control group received no injection, while sham group was injected with saline and experimental groups were given cranberry extract at a dose of 8 and 80 mg/kg respectively by intra peritoneally injections on 7th, 8th and 9th day of gestation. Female mice were killed in 18th day of gestation and not only did embryos examine in terms of apparent abnormalities, but also they were histologically subjected about their liver tissue.
No apparent abnormality in control, sham and 8 mg/kg group was seen at all, whereas in 80 mg/kg group some abnormalities such as micromelia, fetal and placental atrophy and subcutaneous bleeding were observed. The mean of crown- rump length of embryos an placenta diameter in 80 mg/kg group showed a significant decrease in comparison with that of control group. In histomorphometric study of embryos liver in 80 mg/kg group, there were significant increase and decrease in the average of hepatocyte occupancy rate and sinusoids extent percentage rather than that of control group respectively. Also about measurement of hepatocyte nuclei in 80mg/kg group, there was a significant decrease in comparison with that of control group.
Considering the obtained results, it is supposed that prescription of 80 mg/kg cranberry extract to pregnant mouse can cause abnormalities in histological changes in liver tissue and fetal development procedure.