The role of calcium in physiological phenomenona such as sperm acrosome reaction and hyper-activation has been well known. Calcium ionophore A23187 is routinely used for induction of acrosome reaction in mammalians sperm. Therefore, we decided to evaluate the effect of calcium ionophore A23187 on the sperm hyper-active motility patterns.
Materials and methods
Ten semen samples from individuals referring to andrology unit of Isfahan Fertility and Infertility Center (IFIC) were collected for this study. All the samples were processed with DGC (Density Gradient Centrifuging). After induction of acrosome reaction with calcium ionophore A23187, percentage of hyper-activated sperm was assessed using CASA (Computer-Aided Sperm Analysis) system and compared before and after induction of acrosome reaction.
The results of this study showed that percentage of hyper-activated sperm were significantly lower after induction of acrosome reaction compared to before induction of acrosome reaction (P<0.05).
Ca2+ ionophore A23187 increases intracellular Ca2+ concentration. Therefore, sperm are immobilized in the acrosome reaction condition.