P-9: Antioxidant Effects of Vitamin E on Sperm Parameters in Adult Male Mice Treated with Nonyl Phenol
Nonyl phenol (NP) has been considered an endocrine disrupting substance causing reproductive dysfunction and increase in reactive oxygen species production in different organs including testis. vitamin E (VitE) is a potent scavenger of free radicals and is able to prevent the membrane damage mediated by free radicals The aim of this study was to elucidate the protective effects of VitE against reproductive toxicity induced by NP in adult male mice.
Materials and methods
In this study, 32 adult male mice (8-12 weeks) divided in to four experimental groups, control group received corn oil only, NP group received NP (250 mg/ kgbw), VitE group received VitE (100 mg/kgbw), NP plus VitE group received 250 mg/kg NP + 100 mg/kg VitE for 35 days by gavaged. Finally, body and testes weight were recorded and analyzed caudal epididymal spermatozoa.
NP caused a decrease in body and testes relative weights, sperm number and sperm motility compared to control and p-NP+VitE groups. A significant increase was also found in sperm viability in VitE group compared to both NP and control groups. Administration of VitE combined with NP ameliorated these toxic effects.
VitE reduced the oxidative stress toxicity induced by NP in the reproductive system of adult male mice. VitE not only is able to compensate the toxic effects of p-NP on testis weight, sperm number, sperm motility, but also increases sperm viability in adult male mice.