P-2: Protective Effects of Ethyl Pyruvate on Serum Antioxidant Capacity and Testis Germinalepithelium Morphometryin Cyclophosphamide Treated Mice
Cyclophosphamide (CP) is a chemotherapeuticdrug which causes deleterious effects on tissue of testicle and increases free radicals in the body. The aim of this study is to investigate protective effects of ethyl pyruvate (EP) on improvement of testicle inCP treated animals.
Materials and methods
In this study, 15 male mice(6-8 weeks) were divided into 3 groups. Control group receivednormal saline (0.1ml/day, IP), CP group received CP (15 mg/kg/week, IP), and CP+EP group treated with EP (40 mg/kg/day,IP) along with CP. After 35 days, serum total antioxidant capacity (TAC)and samples of testis were used for measurement of the germinal epitheliumthickness and malondialdehyde (MDA) assaying.
This study showed that mean thickness of germinal epithelium in the CP+EP group were higher than those of the CP group (P<0.05). TAC in the CP group was significantly reduced compared with other groups (P<0.05).The MDA levels in the CP group was higher than the two other groups, whereas this increasing was only significant with control group (P<0. 05).
The results of this study showed the role of EP in increasing of serum antioxidantsand improvedgerminal epitheliumthickness and reduced testicular damage and level of MDA.