O-34: Cytoplasmic Transplantation in Oocytes Obtained from Ovarian Tissue Xenotransplantation Lead to Reduced Apoptosis and Higher Fertilization Rate
Although ovarian tissue cryopreservation and transplantation is used for fertility preservation in patient undergoing cytotoxic treatments but obtaining good quality oocyte is still a debate. Xenotransplantation has been introduced as a reliable technique for better understanding of the transplantation conditions, but to achieve healthy embryos, the quality of obtained oocytes need to be improved.
Materials and methods
Mature oocyte were obtained from xenotransplanted human ovarian tissue which were cryopreserved for about 3 years. Cytoplasmic transplantation was done in experimental group amongst good oocytes and those which had impaired quality. Mechanisms involved after cytoplasmic transplantation in improving the oocyte quality in recipient oocytes were studied (n=138).
Cytoplasm transfer from healthy donor oocyte significantly improved the mitochondrial activity leading to reanimation of the recipient oocyte and improving the quality, fertilization rate (P<0.01) and blastocyst formation rate (P<0.01). Our findings also showed that ATM mediated double strand DNA break repair was significantly improved after cytoplasmic transplantation.
Cytoplasmic transplantation after grafting human ovarian tissue can improve the outcome of the treatment and can be used for further exploration of the mechanisms involved in oocyte aging and poor developmental capacity