Past Issue

Volume 9, Supplement 1, Summer 2015 (Presented at 16th Congress on Reproductive Biomedicine and 10th Royan Nursing and Midwifery Seminar) Pages: 30-31

O-13: Evaluation of Leptin Concentration in Seminal Plasma and Its Correlation with Reactive Oxygen Species, Lipid Peroxidation and Pronuclear Formation in Candidates for ICSI Treatment


Background
Leptin, as a key hormone in energy homeostasis, plays an important role in reproduction and male fertility. However, its negative or positive effect on sperm quality and assisted reproduction technique is still controversial. The aim of this study was the comparison of seminal leptin concentration in selected couples with male and female factor for intracytoplasmic microinjection (ICSI) and its correlation with semen quality, oxidative stress, and pronuclear formation.
Materials and methods
Semen samples were obtained from 94 infertile couples (74 male factor and 20 female factor) who were candidate for ICSI treatment in Royan institute. Following classical semen analysis, seminal plasma was separated for assessment of leptin concentration and lipid peroxidation (LPO).Chemiluminescence was employed for reactive oxygen species (ROS) measurement. The formations about pronuclear (PN) and cleavage rate were also recorded after ICSI.
Results
Although, seminal leptin concentration of female factor patients was higher than male factor (P<0.01), lower amount of ROS (P<0.01) significantly was observed in this group. In spite of these outcomes, seminal leptin concentration indicated a positive correlation with LPO in male factor group (P<0.01) and motility in female factor group (P=0.04). Moreover, no correlation was found between seminal leptin concentration and pronuclear formation after ICSI.
Conclusion
This study indicates that seminal leptin leads to sperm lipid peroxidation in infertile male and it could be used as a marker of oxidative stress assessment in semen.